Keywords
angiogenesis
skin wound
How to Cite
Abstract
Background. Skin wound healing is a serious medical problem that is complicated by bacterial infections and requires appropriate care and treatment.
Objective: to study the effect of a highly mineralized spray solution on the regenerative processes of the skin in a wound defect in an experiment.
Materials and Methods. The study was conducted under laboratory conditions on 5 adult Chinchilla rabbits (weight 3.2-3.8 kg). A 10x10 cm area of skin on the back was shaved, and skin fragments measuring 2x2.5 cm were symmetrically excised on both sides of the spine at the level of X-XII ribs. A 0.5 ml solution of Staphylococcus aureus culture concentrate prepared in the laboratory of microbiology of the SI “Institute of Traumatology and Orthopedics of NAMS of Ukraine” was applied to the wounds. On the second and subsequent days of the experiment, a highly mineralized spray solution was applied to the experimental wound, while a physiological saline solution was applied to the control wound. On the 3rd and 7th days, a microbiological culture was taken from the wounds. After 7 days, the skin wound defect was taken for histological examination.
Results. Macroscopically, there was a significant reduction in the area of the wound defect during the first three days of the experiment due to contraction, with a tendency to accelerate in the experimental defects. However, complete closure of the wound did not occur on the 7th day. The results of the cultures confirmed the growth of microbial colonies of Staphylococcus aureus, which visually (growth dynamics, number and size of colonies) did not differ from the biological samples obtained from control and experimental skin defects. Despite the contamination, purulent discharge from the wound was not observed.
Conclusions. The experiment showed that the use of a highmineralized solution-based agent potentially contributes to regenerative processes in the skin, including angiogenesis and dermal restoration, but other effects, such as antiinflammatory and antibacterial, require further research.
References
Zegadło K, Gieroń M, Żarnowiec P, Durlik-Popińska K, Kręcisz B, Kaca W, Czerwonka G. Bacterial Motility and Its Role in Skin and Wound Infections. Int J Mol Sci. 2023 Jan 15;24(2):1707. doi: 10.3390/ijms24021707. PMID: 36675220; PMCID: PMC9864740.
Benito-Martínez S, Pérez-Köhler B, Rodríguez M, Izco JM, Recalde JI, Pascual G. Wound Healing Modulation through the Local Application of Powder Collagen-Derived Treatments in an Excisional Cutaneous Murine Model. Biomedicines 2022, 10, 960. doi: 10.3390/biomedicines10050960.
Russotto V, Cortegiani A, Fasciana T, Iozzo P, Raineri SM, Gregoretti C, Giammanco A, Giarratano A. What healthcare workers should know about environmental bacterial contamination in the intensive care unit. Biomed. Res. Int. 2017. doi: 10.1155/2017/6905450.
Suleyman G, Alangaden G, Bardossy AC. The Role of Environmental Contamination in the Transmission of Nosocomial Pathogens and Healthcare-Associated Infections. Curr. Infect. Dis. Rep. 2018, 20, 12. doi: 10.1007/s11908-018-0620-2.
Wesgate R, Robertson A, Barrell M, Teska P, Maillard JY. Impact of test protocols and material binding on the efficacy of antimicrobial wipes. J. Hosp. Infect. 2019, 103, e25-e32. doi: 10.1016/j.jhin.2018.09.016.
Gemein S, Gebel J, Christiansen B, Martiny H, Vossebein L, Brill FHH, Decius M, Eggers M, Koburger-Janssen T, Meckel M, et al. Interlaboratory reproducibility of a test method following 4-field test methodology to evaluate the susceptibility of Clostridium difficile spores. J. Hosp. Infect. 2019, 103, 78-84. doi: 10.1016/j.jhin.2019.04.011.
Becker B, Henningsen L, Paulmann D, Bischoff B, Todt D, Steinmann E, Steinmann J, Brill FHH, Steinmann J. Evaluation of the virucidal efficacy of disinfectant wipes with a test method simulating practical conditions. Antimicrob. Resist. Infect. Control 2019. doi: 10.1186/s13756-019-0569-4.
Scalise A. The new formulation of the 0,05% sodium hypochlorite electrolytic solution for cutaneous use: reasons and advantages. AboutOpen Dermatology. 2021;8(1):14-22. doi: 10.33393/ao.2021.2221.
Samidah S, Prihantono, Ahmad M, Jompa J, Rafiah S, Usman AN. The effectiveness of 7% table salt concentration test to increase collagen in the healing process of wound. Gac Sanit. 2021;35 Suppl 2:S199-S201. doi: 10.1016/j.gaceta.2021.07.015.
Diegelmann RF, Ward KR, Carr ME, Bowlin GL. Hemostasis of wound having high pressure blood flow using kaolin and bentonite. Patent U.S. Ser. No. 11/884,363 filed Sep. 12, 2008.
Perez-Amodio S, Rubio N, Vila OF, Navarro-Requena C, Castaño O, Sanchez-Ferrero A, Marti-Munoz J, Alsina-Giber M, Blanco J, Engel E. Polymeric Composite Dressings Containing Calcium-Releasing Nanoparticles Accelerate Wound Healing in Diabetic Mice. Adv Wound Care (New Rochelle). 2021 Jun;10(6):301-316. doi: 10.1089/wound.2020.1206.
Yaping Zhou, Hongyu Li, Jiwei Liu, Yan Xu, Yonglu Wang, Hao Ren, Xueming Li. Acetate chitosan with CaCO3 doping form tough hydrogel for hemostasis and wound healing. Polymers for Advanced Technologies. 2019; 30, Issue1:143-152. doi: 10.1002/pat.4452.
Yi Zheng, Jinfu Wu, Yufang Zhu, Chengtie Wu. Inorganicbased biomaterials for rapid hemostasis and wound healing. Chem. Sci., 2023;14:29-53 doi: 10.1039/D2SC04962G.
Muraveinyk V.S, Maltsev DV, Muraveinyk AV, Tarasevych OM. Rezultaty aprobatsii vysokomineralizovanykh produktiv v klinichnii praktytsi. Imunolohiia ta alerholohiia: nauka i praktyka. 2020;1:17-24. doi: 10.37321/immunology.2020.01-03.
Patent KR102022671B1. Composition for skin wound healing and regeneration comprising mineral ion complex. https://patents.google.com/patent/KR102022671B1/en.
Oliveira H, Catros S, Boiziau C, Siadous R, Marti-Munoz J, Bareille R, Rey S, Castano O, Planell J, Amédée J, Engel E. The proangiogenic potential of a novel calcium releasing biomaterial: Impact on cell recruitment. Acta Biomater. 2016 Jan;29:435-445. doi: 10.1016/j.actbio.2015.10.003. Epub 2015 Oct 9. PMID: 26441126.
Tanaka H, Arai K, Fujino A, Takeda N, Watanabe T, Fuchimoto Y, Kanamori Y. Treatment for hypergranulation at gastrostomy sites with sprinkling salt in paediatric patients. J Wound Care. 2013 Jan;22(1):17-8, 20. doi: 10.12968/ jowc.2013.22.1.17
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